Spatial and temporal distribution of a bioluminescent-marked Pseudomonas putida on soybean root

Auteur(s): Beauchamp, Chantal Jeanne; Kloepper, Joseph W.
Résumé: The ability of rhizobacteria to compete with other microorganisms for root colonization may be critical for its establishment on a root. Over a 6 day period, visualization of the spatial and temporal rhizosphere distribution of a bioluminescent-marked rhizobacterium, Pseudomonas putida, strain GR7.4lux, was examined on soybean grown in non-sterile soil conditions. Luminometry technologies showed a rapid root distribution of rhizobacteria where bioluminescence was particularly intense on the seed and upper root parts. The results provide new information on rhizobial root distribution, where, using enrichment broth, 50% of the root tips were still colonized by rhizobacteria up to 6 days after sowing. This suggests that rhizobial enrichment is required to detect low populations at the root tip. Bioluminescent technology represents a promising alternative to previous methods for studying rhizobial growth and distribution on roots
Type de document: Article de recherche
Date de publication: 1 novembre 2003
Date de la mise en libre accès: Accès restreint
Version du document: VoR
Lien permanent: http://hdl.handle.net/20.500.11794/7468
Ce document a été publié dans: Luminescence, Vol. 18 (6), 346–351 (2003)
https://doi.org/10.1002/bio.747
Wiley Interscience
Autre version disponible: 10.1002/bio.747
14694425
Collection :Articles publiés dans des revues avec comité de lecture

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