Effet de paramètres biotiques et abiotiques sur la production et la dégradation de l'ADN environnemental

Authors: Caza-Allard, Isabeau
Advisor: Bernatchez, LouisApril, Julien
Abstract: Environmental DNA (eDNA) is a very promising approach to facilitate and improve the aquatic species monitoring, which is crucial for their management and conservation. In comparison with the plethora of monitoring studies in the fields, relatively few studies have focused on experimentally investigating the "ecology" of eDNA, in particular pertaining to processes influencing the detection of eDNA. The paucity of knowledge about its ecology hampers the use of eDNA analysis to its full potential. In this study, we experimentally evaluated the impact of several biotic and abiotic factors on the rate of production and degradation of eDNA. Individuals of three freshwater fish species (Brown Bullhead, Tench and Yellow Perch) with distinct ecology were placed in two types of water from the St. Lawrence River (Québec, Canada) with very distinct physicochemical characteristics and at three different temperatures. Water samples were then filtered at predetermined time intervals and quantitative PCR was used to quantify the eDNA in each sample. We found that temperature, species, water types, and some interactions between these factors hada strong effect on the production and degradation of eDNA. The results of this study enhance our knowledge about the ecology of eDNA, thus improving eDNA data interpretation.
Document Type: Mémoire de maîtrise
Issue Date: 2021
Open Access Date: 13 December 2021
Permalink: http://hdl.handle.net/20.500.11794/71183
Grantor: Université Laval
Collection:Thèses et mémoires

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