Studies on the internalization mechanisms of cationic cell-penetrating peptides.

Authors: Drin, Guillaume; Carrondo Cottin, Sylvine; Blanc, Emmanuelle; Rees, Anthony R.; Temsamani, Jamal
Abstract: A great deal of data has been amassed suggesting that cationic peptides are able to translocate into eucaryotic cells in a temperature-independent manner. Although such peptides are widely used to promote the intracellular delivery of bioactive molecules, the mechanism by which this cell-penetrating activity occurs still remains unclear. Here, we present an in vitro study of the cellular uptake of peptides, originally deriving from protegrin (the SynB peptide vectors), that have also been shown to enhance the transport of drugs across the blood-brain barrier. In parallel, we have examined the internalization process of two lipid-interacting peptides, SynB5 and pAntp-(43–58), the latter corresponding to the translocating segment of the Antennapedia homeodomain. We report a quantitative study of the time- and dose-dependence of internalization and demonstrate that these peptides accumulate inside vesicular structures. Furthermore, we have examined the role of endocytotic pathways in this process using a variety of metabolic and endocytosis inhibitors. We show that the internalization of these peptides is a temperatureand energy-dependent process and that endosomal transport is a key component of the mechanism. Altogether, our results suggest that SynB and pAntp-(43–58) peptides penetrate into cells by an adsorptive-mediated endocytosis process rather than temperature-independent translocation.
Document Type: Article de recherche
Issue Date: 3 June 2003
Open Access Date: 19 October 2021
Document version: VoR
Creative Commons Licence:
This document was published in: Journal of biological chemistry, Vol. 278 (33), 31192-31201 (2003)
Alternative version: 10.1074/jbc.M303938200
Collection:Articles publiés dans des revues avec comité de lecture

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