Étude du réseau de projections transzonales durant la folliculogenèse et de la méthylation dans la stabilisation des ARN messagers
|Advisor:||Robert, Claude; Delbes, Géraldine|
|Abstract:||Oogenesis, which occurs within the ovarian follicle, is a process closely link tofolliculogenesis. The oocyte growth mainly involves the establishment of a communication network between the gamete and surrounding somatic cells. During the early stages ofoocyte growth, the gamete accumulates mRNAs in its cytoplasm to support maturation and first cell division during transcriptional silencing until the embryonic genome activation.This period can last from days to weeks depending on the species requiring long stability from maternal mRNAs. It is known that communication between oocyte and somatic cellsand use of mRNA reserves are essential for oocyte competence acquisition to sustain fertilization and early embryogenesis. Chemical modifications known to have stabilizing role, such as methylation, have also been detected on the mRNAs. The main hypothesis is the chemical modifications of mRNA, by the addition of methyl groups, that are involved in stabilizing and managing transcripts within oocyte during transcriptional silencing. The communication network could have a role in enabling proteins transfer from cumulus cells allowing mRNA methylation. Objectives are to detectvarious chemical modifications taking place in oocyte transcriptome and to locate and characterize proteins having role of managing the chemical modifications identified on RNA. Many post-transcriptional modifications have been detected within the oocyte and results have shown that m6A and m5C are modifications with higher abundant expression. Proteins characterization involved in these post-transcriptional modifications in ovary and oocyteof mouse, swine and bovine showed methylation activity during folliculogenesis and oogenesis. More research is needed to improve understanding of mechanisms involved inoocyte competences acquisition, but the present study has contributed to the advancement of this knowledge.|
|Document Type:||Mémoire de maîtrise|
|Open Access Date:||12 July 2021|
|Collection:||Thèses et mémoires|
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