Mise au point de la méthode d'ADN environnemental pour les inventaires de salamandres de ruisseaux au Québec

Authors: Plante, Félix
Advisor: Bernatchez, Louis
Abstract: Amphibians have been showing worrying signs of decline on all continents for several years now. In this context, the acquisition of knowledge regarding these species is an essential step leading to good conservation measures. Environmental DNA is an emerging survey method that is rapidly gaining in popularity and testing this method with several species remains an important step in order to better understand its limitations and benefits. This research aims to explore this method with three species of stream-dwelling salamanders in Quebec (Gyrinophilus porphyriticus, Desmognathus fuscus and Eurycea bislineata), with the objective of evaluating its effectiveness with this group of species of high interest from a conservation point of view. Hence, the eDNA method was compared to the traditional method of active stream search for detection (presence/absence of species) as well as for the quantitative aspect. For all three species, results show that eDNA was detected in all streams where the species was observed by active search. In addition, eDNA was detected in nine streams where the species were not detected by active search. The results regarding the quantitative nature of eDNA are not very conclusive, as the relationship between eDNA concentration and salamander density was weak to non-existent with our model. This result is not surprising considering the high variability of the eDNA concentration data. Thus, we conclude that the eDNA method can be a very valuable tool for the detection of these three salamander species. Since the quantitative aspect of eDNA does not seem to be useful, the future of stream-dwelling salamander surveys seems to lie in a shared use of both methods, so as to make the most of the strengths of each and avoid their weaknesses.
Document Type: Mémoire de maîtrise
Issue Date: 2021
Open Access Date: 10 May 2021
Permalink: http://hdl.handle.net/20.500.11794/69064
Grantor: Université Laval
Collection:Thèses et mémoires

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