Differential methylation of inflammatory and insulinotropic genes after metabolic surgery in women

Authors: Vohl, Marie-ClaudeGuénard, FrédéricTchernof, André; Deshaies, Yves; Cianflone, Katherine M.; Kral, John G.; Marceau, Picard
Abstract: Context: Biliopancreatic diversion with duodenal switch (BPD-DS), a metabolic bariatric operation, induces durable loss of excess weight and reduced cardiometabolic risk. Altered epigenetic marks are mechanistically associated with environment-driven phenotypic variations. Objective: The current study aimed to compare gene methylation levels before and after BPD-DS to identify epigenetic marks potentially linked to metabolic improvements induced by BPD-DS. Design and patients: Metabolic risk factors and gene methylation levels of 20 women studied mean 12 years (range 4-22) after BPD-DS were compared to those of 20 severely obese surgical candidates as controls, matched for pre-surgical age, body mass index and dyslipidemia and hypertension prevalences. Whole-genome blood DNA methylation analysis enabled between-group differential methylation analyses. We calculated correlations between methylation levels of the most differentially methylated CpG sites and plasma glucose and insulin levels and HOMA-IR. Results: Differential methylation analysis identified 15,343 genes demonstrating at least one differentially methylated CpG site (p<1.43x10-7). “Diabetic” and “inflammation/immune” functions were among the most overrepresented from the 200 genes exhibiting the largest group differences in methylation levels. CpG sites methylation levels of genes related to insulin action correlated significantly with fasting insulin levels and homeostatic model of insulin resistance (p≤0.002 for all). Conclusion: These findings suggest that differential methylation levels in obese controls versus treated women may partially explain the durable metabolic improvements after BPD-DS.
Document Type: Article de recherche
Issue Date: 3 October 2015
Open Access Date: 30 April 2021
Document version: VoR
Creative Commons Licence: https://creativecommons.org/licenses/by/4.0
Permalink: http://hdl.handle.net/20.500.11794/68964
This document was published in: Journal of clinical epigenetics, Vol. 1 (1), 1-9 (2016)
iMed Pub LLC
Collection:Articles publiés dans des revues avec comité de lecture

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