Differential methylation in glucoregulatory genes of offspring born before vs. after maternal gastrointestinal bypass surgery

Authors: Guénard, Frédéric; Deshaies, Yves; Cianflone, Katherine M.; Kral, John G.; Marceau, PicardVohl, Marie-Claude
Abstract: Obesity and overnutrition during pregnancy affect fetal programming of adult disease. Children born after maternal bariatric gastrointestinal bypass surgery (AMS) are less obese and exhibit improved cardiometabolic risk profiles carried into adulthood compared with siblings born before maternal surgery (BMS). This study was designed to analyze the impact of maternal weight loss surgery on methylation levels of genes involved in cardiometabolic pathways in BMS and AMS offspring. Differential methylation analysis between a sibling cohort of 25 BMS and 25 AMS (2–25 y-old) offspring from 20 mothers was conducted to identify biological functions and pathways potentially involved in the improved cardiometabolic profile found in AMS compared with BMS offspring. Links between gene methylation and expression levels were assessed by correlating genomic findings with plasma markers of insulin resistance (fasting insulin and homeostatic model of insulin resistance). A total of 5,698 genes were differentially methylated between BMS and AMS siblings, exhibiting a preponderance of glucoregulatory, inflammatory, and vascular disease genes. Statistically significant correlations between gene methylation levels and gene expression and plasma markers of insulin resistance were consistent with metabolic improvements in AMS offspring, reflected in genes involved in diabetes-related cardiometabolic pathways. This unique clinical study demonstrates that effective treatment of a maternal phenotype is durably detectable in the methylome and transcriptome of subsequent offspring.
Document Type: Article de recherche
Issue Date: 9 July 2013
Open Access Date: Restricted access
Document version: VoR
Permalink: http://hdl.handle.net/20.500.11794/68963
This document was published in: Proceedings of The National Academy of Sciences of The United States of America, Vol. 110 (28), 11439-44 (2013)
https://doi.org/10.1073/pnas.1216959110
The Academy
Alternative version: 10.1073/pnas.1216959110
23716672
Collection:Articles publiés dans des revues avec comité de lecture

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