Rapid and concomitant gut microbiota and endocannabinoidome response to diet-induced obesity in mice

Authors: Lacroix, SébastienPechereau, FlorentLeblanc, NadineBoubertakh, BesmaHoude, AlainMartin, CyrilFlamand, NicolasSilvestri, CristoforoRaymond, FrédéricDi Marzo, VincenzoVeilleux, Alain
Abstract: The intestinal microbiota and the expanded endocannabinoid (eCB) system, or endocannabinoidome (eCBome), have both been implicated in diet-induced obesity and dysmetabolism. These systems were recently suggested to interact during the development of obesity. We aimed at identifying the potential interactions between gut microbiota composition and the eCBome during the establishment of diet-induced obesity and metabolic complications. Male mice were fed a high-fat, high-sucrose (HFHS) diet for 56 days to assess jejunum, ileum, and cecum microbiomes by 16S rRNA gene metataxonomics as well as ileum and plasma eCBome by targeted liquid chromatography-tandem mass spectrometry (LC-MS/MS). The HFHS diet induced early (3 days) and persistent glucose intolerance followed by weight gain and hyperinsulinemia. Concomitantly, it induced the elevation of the two eCBs, anandamide, in both ileum and plasma, and 2-arachidonoyl-glycerol, in plasma, as well as alterations in several other N-acylethanolamines and 2-acylglycerols. It also promoted segment-specific changes in the relative abundance of several genera in intestinal microbiota, some of which were observed as early as 3 days following HFHS diet. Weight-independent correlations were found between the relative abundances of, among others, Barnesiella, Eubacterium, Adlercreutzia, Parasutterella, Propionibacterium, Enterococcus, and Methylobacterium and the concentrations of anandamide and the anti-inflammatory eCBome mediator N-docosahexaenoyl-ethanolamine. This study highlights for the first time the existence of potential interactions between the eCBome, an endogenous system of multifunctional signaling lipids, and several intestinal genera during early and late HFHS-induced dysmetabolic events, with potential impact on the host capability of adapting to increased intake of fat and sucrose.
Document Type: Article de recherche
Issue Date: 17 December 2019
Open Access Date: 20 December 2019
Document version: VoR
Creative Commons Licence: https://creativecommons.org/licenses/by/4.0
Permalink: http://hdl.handle.net/20.500.11794/37640
This document was published in: mSystem, Vol. 4 (6), (2019)
American Society for Microbiology
Alternative version: 10.1128/mSystems.00407-19
Collection:Articles publiés dans des revues avec comité de lecture

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