Caractérisation du plasmidome complexe d'Aeromonas salmonicida ssp. salmonicida par séquençage à longues lectures
|Advisor:||Charette, Steve; Frenette, Michel|
|Abstract:||Aeromonas salmonicida subsp. salmonicida is an aquatic pathogen that causes furunculosis to salmonids, especially in fish farms. Antibiotherapy is a common method used to treat this worldwide disease. Unfortunately, its effectiveness is becoming limited due to the presence of drug-resistant strains and even multidrug-resistance strains of the bacterium. The study of these bacterial strains becomes necessary in order to identify the genetic elements responsible for this resistance and to understand how they contribute to the spread of antibiotic resistance. In this study, we focused on the A. salmonicida subsp. salmonicida strain SHY16-3432 to characterize novel genetic elements conferring resistance to antibiotics using long-read sequencing technologies. It allowed us to identify three novel plasmid variants named pAsa9b, pAsa5-3432 and pRAS3-3432, the latter two being responsible for the observed antibiotic resistance. The sequence analysis of these three variants revealed that they all differ from their classical counterparts through the presence or absence of mobile genetic elements. The plasmid pAsa5-3432 carries a new multi-drug resistance region composed of numerous mobile genetics elements that appears to have been acquired through plasmid recombination. As for pRAS3-3432, it contains a new inserted element that has only been reported in the swine pathogen Chlamydia suis. Lastly, the only variation between pAsa9b and the reference plasmid is the absence of an insertion sequence in pAsa9b. Overall, these discoveries highlight the significant implication of mobile genetics elements in the genomic plasticity of A. salmonicida subsp. salmonicida and suggest that this aquatic bacterium has a high capacity to interact with other bacteria, including animal pathogens. Furthermore, the data obtained suggest that the use of long-read sequencing technologies is required to fully decipher the genome of bacteria possessing complex plasmid repertoire, such as A. salmonicida subsp. salmonicida.|
|Document Type:||Mémoire de maîtrise|
|Open Access Date:||11 December 2019|
|Collection:||Thèses et mémoires|
All documents in CorpusUL are protected by Copyright Act of Canada.