Mesure par microscopie holographique numérique des propriétés viscoélastiques des cellules entières

Authors: Bilodeau, Philippe
Advisor: Bélanger, ErikMarquet, Pierre
Abstract: The study of viscoelastic properties of whole-cell by optical microscopy allows one to obtain unique information on cell features. It is all the more important to assess those properties all along cultured cell maturation to extract information on its development and health. However, a vast majority of imaging techniques require a marking agent, whilst methods to measure viscoelastic properties are equally invasive. The use of digital holographic microscopy is proposed, since this method allows to image cell culture in real-time without a marking technique and provides quantitative images. Moreover, digital holographic microscopy provides screening deformation at nanoscopic scale induced on cells, without physical contact between the cells and an external instrument. The goal of this project is to develop shear flow assays allowing precise and non-invasive measurements of whole-cell viscoelastic properties. Cell deformation responses caused by the fluid shear stress are interpreted by viscoelastic models where rigidity and viscosity constants are extracted for the whole cell culture simultaneously. Results have shown that shear flow assays allow non-invasive whole-cell measurements of viscoelastic properties. A significant difference between cell properties of NIH 3T3, HEK 293T/17 and neurons have been found. The rigidity constant E1 and the viscosity constant h2 from Standard and Burgers models are viscoelastic properties to be used to discriminate those cell type.
Document Type: Mémoire de maîtrise
Issue Date: 2019
Open Access Date: 11 December 2019
Grantor: Université Laval
Collection:Thèses et mémoires

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