Validation d'échantillonneurs d'air et biais sur la diversité

Authors: Lemieux, Joanie
Advisor: Duchaine, Caroline
Abstract: Different types of air samplers are available to harvest bioaerosols. They all have their advantages and disadvantages, but of these types, one in particular is likely to introduce bias in the treatment and analysis of the results. Liquid-based samplers see a portion of their collection fluid evaporate during operation, which would favor either the loss of bioaerosols by re-aerosolization or their concentration in the fluid. Very little knowledge is known about re-aerosolization, concentration and their effects on results. The main purpose of this study was to document how the evaporation of the collection fluid impacts air sampling. In vitro experiments, in which collection vessels from two liquid samplers (Coriolisμ® and BioSampler®) were inoculated with known bacterial consortia, concluded that reaerosolization and concentration are complex phenomena. Indeed, they are difficult to predict and seem influenced by evaporation, the bacterial genus, the hydrophobicity of the bacterial membrane, the interaction with other bacteria, the composition of the collection fluid, the flow and capture mechanism of the sampler. In addition, experiments in a natural environment have made it possible to compare the diversity harvested by the liquid-based and filter-based samplers by high throughput sequencing methods. One of the peculiarities of this study was that a cassette containing a filter was connected to the BioSampler® air outlet to collect the re-aerosolized bacteria during sampling. The results are unequivocal, several bacterial genera are totally re-aerosolized from the BioSampler® collection vessel. More than half of the bacterial genera harvested by the Coriolisμ® differ from those of the BioSampler®, and vice versa. The filter samplers both harvested a similar bacterial diversity. These results are an important contribution to the scientific field since they prove the biases induced by liquid type samplers.
Document Type: Mémoire de maîtrise
Issue Date: 2019
Open Access Date: 30 September 2019
Permalink: http://hdl.handle.net/20.500.11794/36720
Grantor: Université Laval
Collection:Thèses et mémoires

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