Propriétés antibactériennes envers Enterococcus faecalis et innocuité de quatre composés naturels et de la nisine

Authors: Marcoux, Eve
Advisor: Gauthier, PhilippeGrenier, Daniel
Other Title(s): Propriétés antibactériennes envers Enterococcus faecalis et innocuité de 4 composés naturels et de la nisine
Abstract: To date, no endodontic irrigating solution possessing all ideal characteristics has been developed. Thus, despite different existing decontamination strategies, some bacterial cells organized as biofilm can survive and contribute to the persistence of the infection. In addition, some currently available solutions demonstrate a considerable cytotoxic potential if forced into periapical tissues. The purpose of this study was to investigate new molecules, including nisin, cinnamon oil and polyphenols derived from licorice (licochalcone A, licoricidin and glabridin) for their antimicrobial properties against Enterococcus faecalis and their biocompatibility with human cells. Microplate dilution assays were performed to determine the antibacterial properties of the compounds and their effects on biofilm formation. The synergistic interaction between the compounds was evaluated using the checkerboard technique. The biofilm viabilitywas measured by a bioluminescence assay while biocompatibility and cell proliferation were determined with a colorimetric test measuring cellular respiration. Cinnamon oil has been shown to be the most effective antimicrobial agent against E.faecalis. All compounds tested in the study caused a decrease of biofilm formation proportionalto the reduction in bacterial growth. Synergistic antibacterial effects between nisin and other products have also been observed against E. faecalis. Tested products demonstrated weak or no cytotoxicity towards three oral cell lines. This study provides evidence that nisin, cinnamon oil and licorice-derived polyphenols, more specifically when used in association, may represent promising molecules for root canal disinfection.
Document Type: Mémoire de maîtrise
Issue Date: 2019
Open Access Date: 12 July 2019
Permalink: http://hdl.handle.net/20.500.11794/35461
Grantor: Université Laval
Collection:Thèses et mémoires

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