Validation de la neuraminidase à titre de marqueur enzymatique pour la détection de virus dans l’air

Authors: Toulouse, Marie-Josée
Advisor: Duchaine, Caroline
Abstract: The known methods for the detection of viruses in the air are complex and do not allow to quickly detect several types of viruses at the same time. In addition, little information is available regarding the effectiveness of samplers to collect and preserve the infectivity of the viruses. This project aimed to investigate the detection of viruses in the air based on the presence of an enzymatic label, the neuraminidase. A purified enzyme and strains of influenza viruses were used as models. The effectiveness of three samplers to collect the viruses in the air and was compared and the effects of aerosolization and sampling in two aerosolization chambers on neuraminidase activity and viruses infectivity were studied. The results obtained demonstrate the stability of the neuraminidase and validate its use as an enzymatic marker for the generic, simple, fast and affordable detection of viruses in air samples.
Document Type: Mémoire de maîtrise
Issue Date: 2019
Open Access Date: 10 July 2019
Grantor: Université Laval
Collection:Thèses et mémoires

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