Existence of a microRNA pathway in anucleate platelets

Authors: Landry, PatriciaPlante, IsabelleOuellet, DominiquePerron, Marjorie; Rousseau, Guy; Provost, Patrick
Abstract: Platelets have a crucial role in the maintenance of hemostasis as well as in thrombosis and vessel occlusion, which underlie stroke and acute coronary syndromes. Anucleate platelets contain mRNAs and are capable of protein synthesis, raising the issue of how these mRNAs are regulated. Here we show that human platelets harbor an abundant and diverse array of microRNAs (miRNAs), which are known as key regulators of mRNA translation in other cell types. Further analyses revealed that platelets contain the Dicer and Argonaute 2 (Ago2) complexes, which function in the processing of exogenous miRNA precursors and the control of specific reporter transcripts, respectively. Detection of the receptor P2Y₁₂ mRNA in Ago2 immunoprecipitates suggests that P2Y₁₂ expression may be subjected to miRNA control in human platelets. Our study lends an additional level of complexity to the control of gene expression in these anucleate elements of the cardiovascular system.
Document Type: Article de recherche
Issue Date: 9 August 2009
Open Access Date: Restricted access
Document version: VoR
Permalink: http://hdl.handle.net/20.500.11794/34273
This document was published in: Nature Structural and Molecular Biology, Vol. 16 (9), 961-966 (2009)
http://dx.doi.org/10.1038/nsmb.1651
Nature
Alternative version: 10.1038/nsmb.1651
19668211
Collection:Articles publiés dans des revues avec comité de lecture

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