The repertoire and features of human platelet microRNAs

Élément Dublin CoreValeurLangue
dc.contributor.authorPlé, Hélène-
dc.contributor.authorLandry, Patricia-
dc.contributor.authorBenham, Ashley-
dc.contributor.authorCoarfa, Cristian-
dc.contributor.authorGunaratne, Preethi H.-
dc.contributor.authorProvost, Patrick-
dc.description.abstractPlaying a central role in the maintenance of hemostasis as well as in thrombotic disorders, platelets contain a relatively diverse messenger RNA (mRNA) transcriptome as well as functional mRNA-regulatory microRNAs, suggesting that platelet mRNAs may be regulated by microRNAs. Here, we elucidated the complete repertoire and features of human platelet microRNAs by high-throughput sequencing. More than 492 different mature microRNAs were detected in human platelets, whereas the list of known human microRNAs was expanded further by the discovery of 40 novel microRNA sequences. As in nucleated cells, platelet microRNAs bear signs of post-transcriptional modifications, mainly terminal adenylation and uridylation. In vitro enzymatic assays demonstrated the ability of human platelets to uridylate microRNAs, which correlated with the presence of the uridyltransferase enzyme TUT4. We also detected numerous microRNA isoforms (isomiRs) resulting from imprecise Drosha and/or Dicer processing, in some cases more frequently than the reference microRNA sequence, including 5′ shifted isomiRs with redirected mRNA targeting abilities. This study unveils the existence of a relatively diverse and complex microRNA repertoire in human platelets, and represents a mandatory step towards elucidating the intraplatelet and extraplatelet role, function and importance of platelet
dc.publisherPublic Library of Sciencefr
dc.titleThe repertoire and features of human platelet microRNAsfr
dc.typeCOAR1_1::Texte::Périodique::Revue::Contribution à un journal::Article::Article de recherchefr
dcterms.bibliographicCitationPLoS One, Vol. 7 (12), (2012)fr
dc.subject.rvmSang -- Plaquettesfr
rioxxterms.versionVersion of Recordfr
rioxxterms.project.funder_nameCanadian Blood Servicesfr
rioxxterms.project.funder_nameCanadian Institutes of Health Research Blood Utilization and Conservation
bul.rights.periodeEmbargo0 moisfr
Collection :Articles publiés dans des revues avec comité de lecture

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