Production et charactérisation des anticorps monoclonaux contre le cytomegalovirus

Authors: Al-Halabi, Mohamed Fawaz.
Advisor: Patel, PravinLetarte, Robert
Abstract: Fusions of spleen cells obtained from mice immunized with human cytomegalovirus (CMV) and a mouse myeloma cell line were performed. One monoclonal antibody, CLB-1 was obtained from the four fusions performed. The following aspects of the reaction of the monocolona1 antibody CLB-1, with CMV were investigated: (a) The reactivity and specificity of CLB-1 with CMV; (b) the ability of the CLB-1 monoclonal antibody to detect clinical isolates of CMV; (c ) the kinetics of synthesis of the protein against which the CLB-1 monoclonal antibody reacts ; (d) the ability of the CLB-1 to neutralize CMV; and (e) some characteristics of this monoclonal antibody. The data obtained showed that the CLB-1 monoclonal antibody detected cells infected with CMV A D16 9 , the strain which was used as the immunizing antigen. The CLB-1 antibody showed a high degree cf specificity for the CMV as it did not react with the herpes simplex type-1, herpes simplex type-2 infected cells nor with uninfected human fibroblasts. From the kinetic experiments, the data obtained showed that this monoclonal antibody was directed against a late protein(s) that appeared between 72h and 96h post-infection. The CLB-1 antibody neutralized the virus completely at a dilution of 1:100 of an acitis fluid preparation. Using the double immunodiffusion method, the CLB-1 antibody was found to belong to the IgGl sub-class of immunoglobulins
Reproduction Note: Montréal Trigonix inc. 2018
Document Type: Mémoire de maîtrise
Issue Date: 1986
Open Access Date: 12 February 2019
Grantor: Université Laval
Collection:Thèses et mémoires

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