High definition confocal imaging modalities for the characterization of tissue-engineered substitutes

Authors: Mayrand, DominiqueFradette, Julie
Abstract: Optimal imaging methods are necessary in order to perform a detailed characterization of thick tissue samples from either native or engineered tissues. Tissue-engineered substitutes are featuring increasing complexity including multiple cell types and capillary-like networks. Therefore, technical approaches allowing the visualization of the inner structural organization and cellular composition of tissues are needed. This chapter describes an optical clearing technique which facilitates the detailed characterization of whole-mount samples from skin and adipose tissues (ex vivo tissues and in vitro tissue-engineered substitutes) when combined with spectral confocal microscopy and quantitative analysis on image renderings.
Document Type: Article de recherche
Issue Date: 1 April 2018
Open Access Date: Restricted access
Document version: AM
Permalink: http://hdl.handle.net/20.500.11794/31483
This document was published in: Adipose-Derived Stem Cells, Vol. 1773, 93-105 (2018)
https://doi.org/10.1007%2F978-1-4939-7799-4_8
Springer
Alternative version: 10.1007%2F978-1-4939-7799-4_8
Collection:Articles publiés dans des revues avec comité de lecture

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