Transcriptional response of yellow perch to changes in ambient metalconcentrations—A reciprocal field transplantation experiment

Auteur(s): Bougas, Bérénice; Normandeau, Éric; Grasset, Julie; Defo, Michel Amery; Campbell, P. G. C. (Peter Gerald Cadogan); Couture, Patrice; Bernatchez, Louis
Résumé: Recent local adaptation to pollution has been evidenced in several organisms inhabiting environments heavily contaminated by metals. Nevertheless, the molecular mechanisms underlying adaptation to high metal concentrations are poorly understood, especially in fishes. Yellow perch (Perca flavescens) populations from lakes in the mining area of Rouyn-Noranda (QC, Canada) have been faced with metal contamination for about 90 years. Here, we examine gene transcription patterns of fish reciprocally transplanted between a reference and a metal-contaminated lake and also fish caged in their native lake. After four weeks, 111 genes were differentially transcribed in metal-naïve fish transferred to the metalcontaminated lake, revealing a plastic response to metal exposure. Genes involved in the citric cycle and beta-oxidation pathways were under-transcribed, suggesting a potential strategy to mitigate the effects of metal stress by reducing energy turnover. However, metal-contaminated fish transplanted to the reference lake did not show any transcriptomic response, indicating a reduced plastic response capability to sudden reduction in metal concentrations. Moreover, the transcription of other genes, especially ones involved in energy metabolism, was affected by caging. Overall, our results highlight environmental stress response mechanisms in yellow perch at the transcriptomic level and support a rapid adaptive response to metal exposure through genetic assimilation.
Type de document: Article de recherche
Date de publication: 1 février 2016
Date de la mise en libre accès: Accès restreint
Version du document: VoR
Lien permanent: http://hdl.handle.net/20.500.11794/292
Ce document a été publié dans: Aquatic Toxicology, Vol. 173, 132-142 (2016)
https://doi.org/10.1016/j.aquatox.2015.12.014
Elsevier
Autre version disponible: 10.1016/j.aquatox.2015.12.014
26867186
Collection :Articles publiés dans des revues avec comité de lecture

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