Transcriptional response of yellow perch to changes in ambient metalconcentrations—A reciprocal field transplantation experiment

Authors: Bougas, Bérénice; Normandeau, Éric; Grasset, Julie; Defo, Michel Amery; Campbell, P. G. C. (Peter Gerald Cadogan); Couture, Patrice; Bernatchez, Louis
Abstract: Recent local adaptation to pollution has been evidenced in several organisms inhabiting environments heavily contaminated by metals. Nevertheless, the molecular mechanisms underlying adaptation to high metal concentrations are poorly understood, especially in fishes. Yellow perch (Perca flavescens) populations from lakes in the mining area of Rouyn-Noranda (QC, Canada) have been faced with metal contamination for about 90 years. Here, we examine gene transcription patterns of fish reciprocally transplanted between a reference and a metal-contaminated lake and also fish caged in their native lake. After four weeks, 111 genes were differentially transcribed in metal-naïve fish transferred to the metalcontaminated lake, revealing a plastic response to metal exposure. Genes involved in the citric cycle and beta-oxidation pathways were under-transcribed, suggesting a potential strategy to mitigate the effects of metal stress by reducing energy turnover. However, metal-contaminated fish transplanted to the reference lake did not show any transcriptomic response, indicating a reduced plastic response capability to sudden reduction in metal concentrations. Moreover, the transcription of other genes, especially ones involved in energy metabolism, was affected by caging. Overall, our results highlight environmental stress response mechanisms in yellow perch at the transcriptomic level and support a rapid adaptive response to metal exposure through genetic assimilation.
Document Type: Article de recherche
Issue Date: 1 February 2016
Open Access Date: Restricted access
Document version: VoR
This document was published in: Aquatic Toxicology, Vol. 173, 132-142 (2016)
Alternative version: 10.1016/j.aquatox.2015.12.014
Collection:Articles publiés dans des revues avec comité de lecture

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