Caractérisation d'anticorps anti-ectonucléotidases par immunohistochimie et immunolocalisation de ces enzymes dans le cordon ombilical et dans le rein humain

Authors: Agonsanou, Hervé
Advisor: Sévigny, Jean
Abstract: Ectonucleotidases are families of enzymes present in the cell membrane and whose role is to hydrolyse the nucleotides into nucleosides. The main objective of this study was to characterize antibodies produced in the laboratory against ectonucleotidases and then to evaluate the expression of these enzymes in the umbilical cord and in the kidney by immunohistochemistry. The ectonucleotidases that we have selected for this work were the Nucleosides Triphosphates Diphosphohydrolases (NTPDases) of the cytoplasmic membrane, namely NTPDase1, -2, -3, -8, as well as ecto-5'-Nucleotidase (CD73). In addition, we have performed enzymatic histochemistry to verify the immunolocalization obtained and also to verify if these enzymes are active in the structures detected by immunohistochemistry. Our results suggest that, in the umbilical cord, NTPDase1, -2, -3, and ecto-5'-Nucleotidase are located at the endothelium of the arteries and vein and in Wharton frost. In the kidney, NTPDase1 is located in the blood vessels, NTPDase2 is located on the apical surface of the renal tubules, NTPDase3 as well as NTPDase8 are located in the renal tubules. The current identification of these enzymes at the protein and activity level in the umbilical cord and in the kidney is a prerequisite with the goal to define the role of these enzymes and in extension of extracellular nucleotides in these tissues.
Document Type: Mémoire de maîtrise
Issue Date: 2017
Open Access Date: 24 April 2018
Grantor: Université Laval
Collection:Thèses et mémoires

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