Développement d'une plateforme vaccinale polyvalente basée sur l'utilisation des nanoparticules du virus mosaïque de la papaye (PapMV) et de la transpeptidase Sortase A de Staphylococcus aureus

Authors: Thérien, Ariane
Advisor: Leclerc, Denis
Abstract: Vaccination remains to date the most effective intervention in the prevention and control of infectious diseases. PapMV nanoparticles have shown to be an efficient vaccine platform to increase antigens immunogenicity. While they have shown great potential, the insertion of antigens in the open reading frame (ORF) of the coat protein (CP) can affect its capacity to assemble into nanoparticles. We developed a new method to modify PapMV nanoparticles based on the use of bacterial transpeptidase sortase A (SrtA) to attach antigens directly onto assembled nanoparticles. SrtA attached long antigenic peptides (M2e and T20) onto PapMV nanoparticles without affecting their structural of immunological integrity. These nanoparticles induced strong antigen specific antibodies and fully protected PapMV-SrtA-M2e vaccinated mice against an influenza challenge. The use of the PapMV-SrtA platform will enable the faster and easier development of new vaccines.
Document Type: Mémoire de maîtrise
Issue Date: 2016
Open Access Date: 24 April 2018
Permalink: http://hdl.handle.net/20.500.11794/27635
Grantor: Université Laval
Collection:Thèses et mémoires

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