Étude et développement d'une plateforme microfluidique pour l'imagerie électrochimique

Authors: Kara, Adnane
Advisor: Miled, AmineGreener, Jesse
Abstract: In this project we present a complete microfluidic platform with integrated 200 electrodes for in situ screening and imaging of biochemical samples through a lab-on-chip system. We incorporated electrochemical sensor arrays (20x10) connected to a PCB into a 200 μm tall microfluidic channel. The micro-channel contains three inlets, two of which where used to introduce phosphate buffer saline (PBS), which confined a central stream of ferrocynide solution. A custom multiplexer and potentiostat were used to sequentially perform cyclic voltammetry on each electrode. The behaviour of the system was linear in term of variation of current versus concentration. A pseudo real-time interface collected currents from each electrode. It was then analyzed to detect different species and their concentrations at different locations on-chip. An electrochemical image was generated presenting the concentration distribution inside the microfluidic device. A numerical calculation with COMSOL was achieved to solve Navier-Stocks equation to confirm experimental results, finally microscopic image analyzed to show the position of the confined flow. Key words : Microelectrodes, microfluidics, lab-on-a-chip, electrochemical analysis, printed circuit board, redox reaction.
Document Type: Mémoire de maîtrise
Issue Date: 2015
Open Access Date: 24 April 2018
Permalink: http://hdl.handle.net/20.500.11794/27292
Grantor: Université Laval
Collection:Thèses et mémoires

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