Caractérisation d'un effecteur de phosphoinositides chez le parasite de la malaria Plasmodium falciparum

Authors: Gaumond, David
Advisor: Richard, Dave
Abstract: Malaria is a deadly infectious disease taking more than 500,000 lives each year. The disease is caused by a protozoan of the Plasmodium family. Resistant strains beginning to spread and the inexistence of an efficient vaccine make the discovery of new targets urgent. The parasite secretes proteins to invade the red blood cell. Those proteins are regrouped in the apical complex, a group of organelles used for the invasion. Our research team focus on the transport mechanisms that drive the formation of the apical complex during the cellular division of new parasite. In other terms, we are interested on the role of phosphoinositide in the recruitment of protein inside the Golgi apparatus. After a bioinformatics analyse the P. falciparum genome, we identified many effectors protein that can bind phosphoinositides. Among them, we focused our work on Mal13P1.188, a protein with a Pleckstrin homology domain. We propose that Mal13P1.188 has a role in the recruitment of the apical proteins to the Golgi membrane using phosphoinositide as a marker on the membrane. To verify that hypothesis, we generated a strain of parasite with endogenous Mal13P1.188 tagged to a GFP and a hemagglutinin. With those parasites, we identified Mal13P1.188 near the Golgi apparatus during the Schizont stage of the blood cycle. Other experiment confirmed that the Pleckstrin homology domain of Mal13P1.188 is able to bind different form of phosphoinositides. Finally, more work has to be done to confirm if Mal13P1.188 is essential to the parasite survival.
Document Type: Mémoire de maîtrise
Issue Date: 2016
Open Access Date: 24 April 2018
Grantor: Université Laval
Collection:Thèses et mémoires

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