Characterization of cancer/testis antigen MAGE-A11 for immunotherapy of prostate cancer

Authors: Dadvar, Ehsan
Advisor: Fradet, YvesBergeron, Alain
Abstract: Cancer/testis antigens are ideal targets for cancer immunotherapy because of their limited expression in normal tissues, aberrant expression in malignancies and their immunogenic properties. The aim of this study was to evaluate the potential of cancer/testis antigen, MAGE-A11, as an immunotherapeutic target for development of a prostate cancer vaccine. To accomplish this, we produced the monoclonal antibody 5C4 that is capable of recognizing MAGE-A11 in formalin-fixed paraffin-embedded tissues. We also investigated the expression of MAGE-A11 in a wide variety of cancer cell lines to determine the scope of its expression in cancer. It was shown that MAGE-A11 is widely expressed in malignancies. The highest MAGE-A11 expression was observed in colon cancer and astrocytoma brain tumors. Finally, we identified three naturally processed MHC class II HLA-DR1 epitopes in MAGE-A11 protein, thus confirming its immunogenicity and its potential as a target for cancer immunotherapy.
Document Type: Mémoire de maîtrise
Issue Date: 2014
Open Access Date: 24 April 2018
Permalink: http://hdl.handle.net/20.500.11794/26789
Grantor: Université Laval
Collection:Thèses et mémoires

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