Évaluation de l'impact de pansements biologiques humains produits par génie tissulaire sur la guérison de plaies cutanées murines
|Authors:||Morissette Martin, Pascal|
|Advisor:||Fradette, Julie; Moulin, Véronique|
|Abstract:||Promotion of skin repair for acute or chronic wounds through the use of tissue-engineered products is an active field of research. This study evaluates the effects mediated by two types of tissue-engineered biological dressings containing human in vitro-differentiated adipocytes or adipose-derived stromal cells (ASCs). Re-epithelialization, granulation tissue formation and neovascularization of full-thickness cutaneous wounds were specifically assessed using a murine model featuring a fluorescent epidermis. In comparison to wounds that did not receive either type of biological dressings, treated wounds displayed significantly faster wound closure rates. Non-invasive imaging of GFP-expressing keratinocytes determined that wound closure was independent from re-epithelialization mechanisms, while histological assessments of the scar tissues showed thicker granulation tissues enriched in collagens and increased angiogenesis. Taken together, these results establish that engineered substitutes featuring adipocytes or ASCs can promote cutaneous healing when applied as temporary dressings, suggesting their relevance for chronic wound management studies.|
|Document Type:||Mémoire de maîtrise|
|Open Access Date:||24 April 2018|
|Collection:||Thèses et mémoires|
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