Étude des mécanismes moléculaires précoces de la réponse neuro-immunitaire dans des modèles de la maladie de Parkinson
|Abstract:||This thesis focuses on the study of the mechanisms of action of the neurotoxin MPTP and the process leading to innate immune cells activation. In the first part, a study of the chronology of immune events taking place in this experimental context was performed using the transgenic mice cis-NF-ĸBeGFP, CX3CR1GFP and lysMeGFP showing very early inflammatory activities in the myenteric plexus. In a second phase, a mechanistic study on immune cells ability to carry the toxin was conducted using the fluorescent analog of MPP+, APP+. Immune cells can indeed carry this analog, and therefore may be modulated by the neurotoxin. Finally, in a third aspect, an in vitro conditioning assay with monocytes and dopaminergic neurons showed the importance of a combination of the effect of MPP+ on dopaminergic neurons and a pro-inflammatory environment to generate alterations in these neurons.|
|Document Type:||Mémoire de maîtrise|
|Open Access Date:||23 April 2018|
|Collection:||Thèses et mémoires|
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