Distribution chez la souris gestante du transgène GFP introduit avec un virus adéno-associé du sérotype 9
|Authors:||Kelu, Ken Bisabu|
|Abstract:||Gene therapy using viral vectors is one of the therapeutic approaches possible for the development of genetic therapies for monogenic diseases including Friedriech's Ataxia (FRDA). In order to verify the effectiveness of adeno-associated virus (AAV) to transfer a transgene in the tissues they infect, we inserted the gene encoding the enhanced form of the green fluorescent protein (eGFP) in AAV Serotype 9 (AAV9). These recombinant AAV (rAAV) were then administered by intravenous and intraperitoneal to pregnant female mice. The results obtained show that the transgene was transferred in both in the female’ mouse tissues and in those of young mice.|
|Document Type:||Mémoire de maîtrise|
|Open Access Date:||23 April 2018|
|Collection:||Thèses et mémoires|
All documents in CorpusUL are protected by Copyright Act of Canada.