Study the effects of cigarette smoke on gingival epithelial cell growth and the expression of keratins

Authors: Alharbi, Ibrahim
Advisor: Rouabhia, Mahmoud
Abstract: This research project is aimed to evaluate the effect of long-term exposure to cigarette smoke (CS) on the gingival epithelial cells growth and the expression of different keratins (K). The CS-exposed cells are showing high metabolic activity and cell proliferation as compared to non-exposed cells, suggesting an increase in the cells growth. Importantly, the prolonged exposure to CS was found to decrease Bax/Bcl-2 ratio that indicates the death resistance. Moreover, the gene expression analysis showed that the exposure to CS decreases the mRNA level of K5, K1, K10, and K16; excepting K14 and K6 were showing an increase after a certain exposure period. Interestingly, protein production analysis of K5, K1, and K16 are showing a stable expression after the exposure to CS. In contrast, K14 and K6 are significantly increased, while K10 decreased after CS exposure. These observations suggest an abnormal growth of the gingiva that may lead to the periodontal diseases.
Document Type: Mémoire de maîtrise
Issue Date: 2015
Open Access Date: 23 April 2018
Grantor: Université Laval
Collection:Thèses et mémoires

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