L'impact de la surexpression de pré-enképhaline striatale sur la dyskinésie induite par la L-DOPA dans la maladie de Parkinson

Authors: Bergeron-Bezeau, François
Advisor: Samadi, Pershia
Abstract: To address the role of striatal upregulation of opioid peptides in L-DOPA-induced dyskinesia (LID), we used viral vector gene transfer to overexpress pre-enkephalin (pENK) in striatum of unilateral 6-hydroxydopamine mouse model of LID. One week before L-DOPA treatment (3 weeks after dopamine lesion) three groups received either striatal injection of: Saline (Saline); or Adeno-Associated Virus expressing Green Fluorescent Protein (AAV-GFP); or AAV-GFP-pENK. 4th group only received L-DOPA without any striatal injection (NSI), and 5th group did not receive L-DOPA. The severity of dyskinesia after L-DOPA administration was assessed, during 3 weeks. Our results showed an important reduction of LID in GFP- and pENK- injected mice (-70% to -82%) compared with Saline and NSI groups. The level of pENK mRNA was higher in the anterior and posterior parts of lesioned-striatum in pENK and GFP groups, respectively. Our study provides the first evidence that upregulation of striatal pENK mRNA could reduce LID.
Document Type: Mémoire de maîtrise
Issue Date: 2015
Open Access Date: 23 April 2018
Permalink: http://hdl.handle.net/20.500.11794/25725
Grantor: Université Laval
Collection:Thèses et mémoires

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