Développement de nouvelles stratégies de génotypage sanguin à l'aide de nanoparticules composites super luminescentes

Authors: Ratelle, Olivier
Advisor: Boudreau, Denis
Abstract: To ensure patient safety during blood transfusions, blood banks must test donors and receivers to ensure the compatibility of blood. Blood genotyping methods used presently are complex and fastidious and do not allow the complete characterization of all donated blood. This project involves the development of new strategies for blood genotyping using super luminescent composites nanoparticles. Such biosensors could be used to facilitate these operations by providing a method that would avoid the enzymatic amplification step which limits the analysis throughput. In doing so, it would be much faster and cheaper than traditional methods. In this master's essay, the synthesis of core-shell fluorescent nanoparticles, surface functionalization and characterization of the colloids will be presented. Also, the analytical performance of the system developed will be discussed. The nanoparticles are made of a silver core in order to exploit their plasmonic properties. Thereafter, they are covered with a silica layer doped with fluorophores by using a modified Stöber method in order to be stable and fluorescent. Subsequently, DNA single stranded probes are grafted to the surface of these nanoparticles to specifically capture the complementary DNA targets, a task that will be facilitated by the silica surface which provides access to a range of functionalization strategies using silane chemistry. Finally, a polymer transducer is complexed to the DNA probe and allows recognition of hybridization by Förster Resonant Energy Transfer (FRET).
Document Type: Mémoire de maîtrise
Issue Date: 2014
Open Access Date: 20 April 2018
Permalink: http://hdl.handle.net/20.500.11794/25578
Grantor: Université Laval
Collection:Thèses et mémoires

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