Analyse des isomères de F2-isoprostanes par chromatographie liquide couplée à la spectrométrie de masse dans la circulation maternelle en fin de grossesse
|Abstract:||An oxidative stress is defined as an imbalance between the production of reactive oxygen species and antioxidant defenses of the organism. This imbalance has been associated with preeclampsia, a pathology of the mid-to-late pregnancy. Peroxidation of arachidonic acid generates sixty-four isomers of F2-isoprostanes. The latter are recognized as the most reliable biomarkers of oxidative stress in vivo. A method using liquid chromatography tandem mass spectrometry (HPLC-MS/MS) for the determination of seven isomers of F2-isoprostanes in plasma samples, whole blood and erythrocyte membranes has been developed and validated. The F2-isoprostanes correlated positively with several trans fatty acids in plasma at end of the pregnancy. Unexpectedly, F2-isoprostanes were less abundant in preeclampsia than in control pregnancies at the third trimester.|
|Document Type:||Mémoire de maîtrise|
|Open Access Date:||20 April 2018|
|Collection:||Thèses et mémoires|
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