Analyse spatiotemporelle des enzymes de déméthylation de l'adn et des histones dans l'embryon bovin
|Abstract:||In mammals, the transition from one generation to the next requires genomic reprogramming. Such epigenetic change is mediated by paternal and maternal DNA demethylation as well as histone lysines demethylation after fertilization, which is a poorly understood process. Some family of enzymes have recently been associated to those process: the deaminases, like Aicda (activation-induced cytosine deaminase), and Tet (Ten-eleven translocation) dioxygenases, Tet1, Tet2 and Tet3. Many lysine specific histone demethylases (KDM) have been identified in the past few years but little is known about their roles in mammalian embryo. The objective of this study was to develop of a spatiotemporal expression profile of those proteins at different preimplantation stage of bovine embryo. We suggest an active participation of Tet3 in DNA methylation, possibly supported by Tet2 but without Tet1 or Aicda. We also demonstrate the presence and specific localization of KDM3A, KDM4A, KDM4C and KDM5B which may suggest a role during the different embryonic stages. This information opens up the possibilities for further research in order to reduce epigenetic abnormalities associated to assisted reproduction technologies.|
|Document Type:||Mémoire de maîtrise|
|Open Access Date:||19 April 2018|
|Collection:||Thèses et mémoires|
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