Potentiel nutraceutique des constituants du babeurre
|Advisor:||Gauthier, Sylvie; Pouliot, Yves|
|Abstract:||The minor components in milk fat globule membrane (MFGM) are associated with various biological activities. Buttermilk, owing to its obtainment process, is a milk product particularly rich in MFGM components. This feature accounts for the growing interest in this by-product of the manufacturing of butter. The number of scientific articles dedicated to it has quadrupled in the past 20 years (PubMed). Until today the vast majority of tasks involving buttermilk aimed at either dividing or concentrating its various components, primarily, phospholipids. However, the biological effects of all the components in buttermilk before and after their digestion remains poorly understood. Therefore, the purpose of this research was to evaluate the impact that buttermilk components have on the different risk factors associated with cardiovascular disease (CVD). Initially, the results of in vitro model study revealed that buttermilk freshly concentrated by microfiltration (MF) has the potential to reduce the micellar solubility of cholesterol. These conclusive results presaged the capacity of certain components in buttermilk to regulate the intestinal absorption of cholesterol. Despite, their division proved to have a negative impact on the potential insolubilization of the cholesterol previously observed. Then, in a clinical crossover analysis, minor components of buttermilk - most likely phospholipids - demonstrated a capacity to positively influence the lipid profiles of moderately hypercholesterolemic subjects. Moreover, during this same study, the consumption of buttermilk compared with a placebo proved capable of lowering blood pressure in some patients, possibly resulting from its peptide components. That is to say that, in an in vitro model study, peptides derived from the hydrolysis of fresh buttermilk (UF) demonstrated antioxidant potential superior to those obtained from whey (UF) and skim milk. Within the framework of all studies on this project, various biological activities proved almost uninfluenced by the action of heat treatment when products were hydrolyzed following their consumption. The results of this study allow for a better understanding of the beneficial properties of buttermilk and pave the way for new uses of it as an active ingredient. Indeed, this innovative project has allowed us to demonstrate the potential of buttermilk as a functional food rather than as a source of ingredients needing to undergo a purification or concentration process. In this way, it seems that all components in buttermilk play a role in the observed benefits on risk factors of CVD.|
|Document Type:||Thèse de doctorat|
|Open Access Date:||19 April 2018|
|Collection:||Thèses et mémoires|
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