Liaison de la protéine spermatique bovine Spam-1 à la zone pellucide de l'ovule
|Abstract:||Spam-1 is a sperm surface protein that would help the spermatozoa going through the cumulus, disrupting the hyaluronan around the egg. It would also have a role to play in the secondary binding of the spermatozoa to the zona pellucida of the oocyte. The aim of the present study, by the construction of various fluorescent recombinant proteins containing the sequences of the different domains of Spam-1, is to clearly demonstrate that bovine spermatic Spam-1 protein is directly implicated in the secondary binding of the spermatozoa to the zona pellucida and to show that the C-terminal domain is implicated. The results of this study are going to determine the importance of Spam-1 in the spermatozoa binding to the zona pellucida and in getting a better understanding of the mechanisms surrounding the interactions between spermatozoa and oocyte.|
|Document Type:||Mémoire de maîtrise|
|Open Access Date:||19 April 2018|
|Collection:||Thèses et mémoires|
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