Identification des régions de surface des nanoparticules du virus de la mosaïque de la papaye (PAPMV) dans le but d'optimiser son utilisation comme plateforme vaccinale
|Advisor:||Leclerc, Denis; Majeau, Nathalie|
|Abstract:||Surface-exposed regions of Papaya Mosaic Virus (PapMV) were evaluated by two different methods: immunoblot assay and chemical modifications followed by mass spectrometry. Three regions were targeted by both techniques: the N- and C-termini and region 125-136. These regions are therefore considered to be major surface epitopes. Four other regions were only detected by the immunological technique: regions 117-128, 141-152, 173-184 and 189-200. Sites of fusion using the Influenza virus HA11 peptide were evaluated on the PapMV vaccine platform. Fusions of HA11 at three functional sites located after amino acids 12 and 187 and at the C-terminus led to the production of PapMV particles. It was successfully demonstrated that the HA11 epitope was located at the surface of the particles. The stability and immunogenicity of the nanoparticles were evaluated, and a direct correlation was established between the stability of the particles and their ability to trigger an efficient immune response against HA11.|
|Document Type:||Mémoire de maîtrise|
|Open Access Date:||18 April 2018|
|Collection:||Thèses et mémoires|
All documents in CorpusUL are protected by Copyright Act of Canada.