Système de recombineering pour les bactériophages infectant Lactococcus lactis

Authors: Moisan, Maxim
Advisor: Moineau, Sylvain
Abstract: Lactococcus lactis is a widely used bacterium by the dairy industry and its sensitivity to virulent bacteriophages has an important economic relevance. Thus, the characterization of these phages is needed to better understand their mecanism of infection and to control them. Currently, no reliable tool is available to genetically modify virulent lactococcal phages. A possible approach is a genetic engineering method called recombineering which relies on the use of phage recombinases. The aim of this M. Sc. Project was to develop a recombineering system for virulent phages infecting L. lactis. In vivo experiments have shown that recombinases Sak and Sak3 were not efficient for recombineering. Interestingly, a few recombinants were obtained independantly of these recombinases, probably by the uncharacterized process named oligo-recombination. This mechanism had not been observed yet in a Gram-positive bacterium, and it has also been efficient to produce lactococcal phage mutants.
Document Type: Mémoire de maîtrise
Issue Date: 2012
Open Access Date: 18 April 2018
Grantor: Université Laval
Collection:Thèses et mémoires

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