Impact d'une perturbation environnementale sur la dynamique de la structure et de la diversité taxonomique des communautés bactériennes lacustres

Authors: Laplante, Karine
Advisor: Derome, Nicolas
Abstract: There are growing concerns about ecosystems being disturbed by mining activities which release heavy metals consequently exerting a strong selective pressure on the functional diversity of bacterial processes. Therefore, this thesis aimed to characterize the impact of diffuse polymetallic pollution over the taxonomic diversity and structure of lacustrine bacterial communities from sites located in the surroundings of Rouyn-Noranda. To this end, denaturing gradient gel electrophoresis and pyrosequencing of 16S rRNA sequences were used. The results showed that i) communities’ equilibrium was disturbed at both the diversity and structure levels and that ii) a similar selective pressure induced parallel evolution within independent bacterial communities. Our study therefore sheds light on the importance of analyzing bacterial communities at the taxonomic diversity and structure level to generate insights about the community changes taking places under polymetallic contaminants at both an ecological and evolutionary point of view.
Document Type: Mémoire de maîtrise
Issue Date: 2012
Open Access Date: 18 April 2018
Permalink: http://hdl.handle.net/20.500.11794/23506
Grantor: Université Laval
Collection:Thèses et mémoires

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