Génomique fonctionnelle des gènes uniques chez Pseudomonas aeruginosa LESB58 et essentiels à l'infection pulmonaire chronique
|Advisor:||Lévesque, Roger C.|
|Abstract:||Pseudomonas aeruginosa is an opportunistic pathogen and causes chronic pulmonary infection to cystic fibrosis (CF) patients. The hypervirulent epidemic strain LESB58 is associated with high transmissibility and is highly resistant to antibiotics causing increased morbidity and mortality. Whole genome sequencing of LESB58 revealed a 90% highly conserved core genome and 455 additional genes grouped in five genomics islands (GIs) and six prophages (PPs). The aim of this study was to determine the implication of the accessory genome in LESB58 virulence. We performed a signature tagged mutagenesis (STM) followed by an in vivo screening of the mutants. From 162 STM mutants defective for in vivo maintenance, we selected 11 harbouring an insertion in GI or PP for further virulence analysis. Two of these mutants were used for genomics analysis in order to better understand their incapacity for in vivo maintenance in the rat model of chronic lung infection.|
|Document Type:||Mémoire de maîtrise|
|Open Access Date:||18 April 2018|
|Collection:||Thèses et mémoires|
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