Amélioration du taux de greffe de cellules myogéniques pour la dystrophie musculaire de Duchenne : surexpression du récepteur à l'IGF-1 sur des cellules humaines et utilisation du facteur de croissance MGF
|Abstract:||Myogenic cells transplantation is one of the promising therapies to treat dystrophic patients. Early cells death and poor migration are important problems who limit this therapy’s success. MGF-Ct24E, a synthetic copy of the peptide E from the IGF-1 Ec isoform, was used. The peptide increases the proliferation of cells and did no effect on the differentiation. Injected systematically and intramuscularly, MGF-Ct24E increased the graft success. When the cells were pretreated with the peptide and co-injected with it, no increase in the graft success was seen. The overexpression of IGF-1 receptor on human cells with a lentivirus increased the proliferation rate and did no effect on their differentiation. When the cells were selected for a high expression of the IGF-1 receptor and for a high phosphorylation of Akt, the graft success was increased. Together, those results are offering an interesting avenue for the amelioration of cells transplantation in DMD.|
|Document Type:||Mémoire de maîtrise|
|Open Access Date:||13 April 2018|
|Collection:||Thèses et mémoires|
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