Optimisation de la culture d'anthères chez l'orge de printemps à six rangs (Hordeum vulgare)
|Abstract:||Eight hormonal regimes were compared in anther culture on two genotypes of six-row spring barley. Observations were made on four parameters: the number of calli, embryos, green plants and albinos. An analysis of variance showed that the genotype (G), the treatment (T), the harvest rank of the spike (R) and the R X G interaction all had significant impacts on the studied parameters. Altona proved much more productive in anther culture than C97-21-38-3 (19.6 vs 1.3 green plants per 100 anthers). Treatments 3 (0.1 mg/L BAP; 0.1mg/L 2,4-D) and 6 (0.3 mg/L BAP; 0.3 mg/L 2,4-D) yielded between 2 and 3-fold more green plants than the control treatments. Finally, whereas the second and third spikes produced significantly more green plants than the first spike with Altona, no such difference was observed with C97-21-38-3.|
|Document Type:||Mémoire de maîtrise|
|Open Access Date:||13 April 2018|
|Collection:||Thèses et mémoires|
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