L'incorporation de CD154 par le VIH-1 et son effet sur l'activation des macrophages dérivés de monocytes humains
|Advisor:||Tremblay, Michel J.|
|Abstract:||CD154 interacts with CD40 found on antigen-presenting cells such as macrophages and plays a crucial role in activating these cells and initiating immune responses. We have previously reported that CD154 is incorporated within HIV-1 envelope and is effective at activating primary B lymphocytes. In this study, we tested if such CD154-bearing virions are also effective in activating human monocyte-derived-macrophages (MDM). We observed an increase in IL-8 secretion, but it is highly variable depending on viral productions and donors used. The induction of IL-8 production is specific to CD154 since a mutant form does not induce it and is independent of gp120. The production of MMP-1 and -2 is influenced by CD154 on 293T cells but the specificity is lost when viral particles are used. These results suggest a possible way used by the virus to attract target cells to the site of infection and thereby favour its own replication.|
|Document Type:||Mémoire de maîtrise|
|Open Access Date:||13 April 2018|
|Collection:||Thèses et mémoires|
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