Towards all optical functional imaging of neuronal cells : development of a video rate multimodal laser scanning microscope
|Advisor:||Côté, Daniel; Lin, Charles P.|
|Abstract:||The main goal of this master's project is to develop a novel video rate multimodal laser scanning microscope specifically designed to perform functional imaging of neuronal cells. A microscope was designed, built and tested at the Centre de Recherche Universite Laval - Robert Giffard (CRULRG) in Quebec City. This thesis summarizes the system implementation choices we made (optical design, electronics) in order to achieve our imaging goals. Results showing the correlation between the optical signal variations and the cell transmembrane potential or calcic activity changes are presented. This tool opens the door to new experiments, allowing for non-invasive observations of cell activity over a large volume thus overcoming important limitations of the established 'patch-clamp' technique.|
|Document Type:||Mémoire de maîtrise|
|Open Access Date:||13 April 2018|
|Collection:||Thèses et mémoires|
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