Étude d'un système de préfermentation en continu du lait par une culture mixte immobilisée fonctionnelle

Authors: Grattepanche, Franck
Advisor: Lacroix, Christophe
Abstract: The objective of this work was to study an immobilized-cell system for continuous inoculation and prefermentation of milk by a mixed culture containing a nisin Z producer (Lc. diacetylactis UL719) and two nisin-sensitive strains for acidification (Lc. cremoris) and exopolysaccharide production (Lb. rhamnosus RW-9595M). Batch fermentations of milk traditionally inoculated with free-cell mixed culture of the three strains were also performed at different temperatures. In these conditions, we showed that growth of Lc. diacetylactis was stimulated by commensalism, leading to an increase of nisin Z production. Continuous prefermentation of milk with immobilized cells carried out over 3 weeks leads to high inoculation of milk with Lc. diacetylactis and Lb. rhamnosus . In fermented milk, obtained after an additional batch incubation, populations of these two strains remained at high concentrations leading to high nisin production and higher exopolysaccharide concentration compared to traditional batch fermentation of milk. The real-time PCR method, developed in this project, was successfully used to quantify very low cell concentrations of Lc. cremoris in prefermented and fermented milks. Immobilization and continuous culture also lead to important cell physiological adaptations for Lb. rhamnosus and Lc. diacetylactis. Lb. rhamnosus became much more tolerant to nisin Z, and both strains exhibited a large increase in milk acidification capacity.
Document Type: Thèse de doctorat
Issue Date: 2005
Open Access Date: 12 April 2018
Permalink: http://hdl.handle.net/20.500.11794/18847
Grantor: Université Laval
Collection:Thèses et mémoires

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