Identification, caractérisation et utilisation d'un promoteur de la famille des MADS BOX isolé à partir du génome de Medicago sativa, MSMADS1, avec pour objectif l'application d'une stratégie d'ablation florale
|Advisor:||Bilodeau, Pierre; Belzile, François|
|Abstract:||Progress in genetic engineering has led to the development of a new system to produce therapeutic molecules in plants: molecular pharming. The interest to transform plants into molecular factories for human purposes is explained by its lower cost, its biosecurity and the capacity of plants to synthesize recombinant proteins with appropriate folding, glycosylation and activity. Floral ablation represents one of the best ways to establish both male and female genetic confinement in open field conditions. With the final objective to develop this strategy in alfalfa (Medicago sativa), the promoter of a MADS box gene, MsMADS1, was isolated from the alfalfa genome. The genomic organisation of MsMADS1 appears to be unusual with a first intron longer than 1,9 kp without homology to any known sequence. This promoter was fused to the GUS reporter gene and a cytotoxic protein gene (saporin). Following Agrobacterium-mediated transformation of Arabidopsis thaliana (Arabidopsis) with various constructs, transgenic plants were regenerated and analysed. Transient expression was also assayed to evaluate GUS expression in alfalfa cells. Additional MsMADS1 genomic sequence was obtained by genome walking. In transgenic Arabidopsis, the MsMADS1 promoter allowed GUS expression preferentially in flowers. Surprisingly, saporin constructs resulted in the production of viable transgenic plants with a phenotype identical to wild Arabidopsis.|
|Document Type:||Mémoire de maîtrise|
|Open Access Date:||12 April 2018|
|Collection:||Thèses et mémoires|
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