Caractérisation morphologique et cytogénétique de deux lignées d'Arabidopsis thaliana déficientes pour PMS1, une protéine du système de correction des mésappariements

Authors: Bouchard, Éric
Advisor: Belzile, François
Abstract: The eukaryotic DNA mismatch repair system (MMR) is responsible for the repair of replication errors, and plays a role in genetic recombination. AtPMS1, a MMR gene from Arabidopsis thaliana, has already been shown to play a role in DNA repair but its involvement in meiosis has yet to be examined. We characterised two lines of insertional mutants at AtPMS1. A first mutant line has abnormal flowers, which is probably a phenotype unrelated to the loss of AtPMS1 activity but rather caused by cosuppression. The second mutant line shows many phenotypes, likely linked to AtPMS1 inactivation: a 65 to 80% reduction in seed production, an abnormal segregation of the mutant allele and cytogenetic abnormalities in 16% of the microsporocytes (chromosome fragmentation and missegregation). The observed bias in segregation as not been observed in other eukaryotes, and seems to be a particularity of MutL homologues in Arabidopsis or plants as a whole.
Document Type: Mémoire de maîtrise
Issue Date: 2006
Open Access Date: 12 April 2018
Grantor: Université Laval
Collection:Thèses et mémoires

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