Mise en place d'une chaîne d'analyse et de traitement de biopuces
|Abstract:||The goal of this dissertation was to design and implement a microarray analysis pipeline. The first tool of the microarray pipeline is a web-based LIMS: SLIMS. It allows the storage of all data related to experiments and samples from harvest to RNA extraction. This tool was designed in PHP and MySQL allowing easy access and manipulation of the data. A tranfer algorithm was designed to allow stored data to be automatically integrated into the BASE software, a tool that allows storage and analysis of microarray data. An annotation algorithm was also designed in order to annotate genes that are on the microarrays. A lignin/cellwall annotation was also included to enable the rapid indentification of all the genes related to the lignin biosynthesis pathway and cell wall assembly. This pipeline was used to analyze transgenic spruce overexpressing the pine LIM2 gene.|
|Document Type:||Mémoire de maîtrise|
|Open Access Date:||12 April 2018|
|Collection:||Thèses et mémoires|
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