Génomique fonctionnelle in vivo de l'oxydoréductase PA3498 chez Pseudomonas aeruginosa
|Advisor:||Lévesque, Roger C.|
|Abstract:||Pseudomonas aeruginosa is a Gram negative bacteria causing chronic pulmonary infections in cystic fibrosis patients. Virulence factors of this pathogen facilitate the colonization of the pulmonary tract and lungs of cystic fibrosis patients. Our team has developed a PCR-based signature-tagged mutagenesis technique permitting the screening of a collection of 7968 mutants. A total of 214 mutants, representing transposition events into 148 open reading frames, were shown to be attenuated in lung infection and were retained for further analysis. Of these genes, we chose PA3498 for its characterization. We have concluded that this gene is coding for an oxydoreductase sharing homology with a familly of oxydoreductases including PDR protein of Burkholderia cepacia. Finally, PA3498 protein is needed to initiate or/and to maintain the pathogen in vivo and this protein plays a role in the maturation and processing of the P. aeruginosa exoproteases.|
|Document Type:||Mémoire de maîtrise|
|Open Access Date:||11 April 2018|
|Collection:||Thèses et mémoires|
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