Étude du fractionnement par nanofiltration et électronanofiltration de peptides issus de l'hydrolyse enzymatique de la beta-lactoglobuline

Authors: Lapointe, Jean-François
Advisor: Pouliot, YvesGauthier, Sylvie
Abstract: The purpose of this work was to study the peptide fractionation of a tryptic β-lactoglobulin (β-LG) hydrolysate using nanofiltration (NF). More specifically this thesis aimed at determining how the fractionation process was influenced by concentration polarization and fouling, and next, to show if it was possible to increase peptide fractionation by applying an electric field during NF, a process called electrofiltration (EF). The results showed that the polarization of peptides can be used favorably to increase the selective transmission of cationic peptides (basic pI) in the permeate during NF of the tryptic β-LG hydrolysate at basic pH. Under these conditions, the hydraulic resistance associated with fouling of the NF membrane is generally restricted. However, although limited, fouling by tryptic β-LG peptides modify both the sieving and electrostatic properties of the membrane. Peptide-membrane interactions and the nature of interacting peptides vary according to the pH. In this regard, some specific peptides seem to be prevailing in the membrane fouling process. Regarding EF, the purpose-built module developped during this work allowed to increase the separation of cationic peptides, as compared to conventional NF.
Document Type: Thèse de doctorat
Issue Date: 2004
Open Access Date: 11 April 2018
Permalink: http://hdl.handle.net/20.500.11794/17917
Grantor: Université Laval
Collection:Thèses et mémoires

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