17β-estradiol delays 6-OHDA-induced apoptosis by acting on Nur77 translocation from the nucleus to the cytoplasm

DC FieldValueLanguage
dc.contributor.authorRenaud, Justine-
dc.contributor.authorChiasson, Keith-
dc.contributor.authorBournival, Julie-
dc.contributor.authorRouillard, Claude-
dc.contributor.authorMartinoli, Maria-Grazia-
dc.date.accessioned2018-02-13T13:53:46Z-
dc.date.available9999-12-31-
dc.date.issued2013-11-26-
dc.identifier.issn1029-8428fr
dc.identifier.urihttp://hdl.handle.net/20.500.11794/17041-
dc.description.abstractNuclear receptors (Nurs) represent a large family of gene expression regulating proteins. Gathering evidence indicates an important role for Nurs as transcription factors in dopamine neurotransmission. Nur77, a member of the Nur superfamily, plays a role in mediating the effects of antiparkinsonian and neuroleptic drugs. Besides, Nur77 survival and apoptotic roles depend largely on its subcellular localization. Estrogens are known for their neuroprotective properties, as demonstrated in animal and clinical studies. However, their action on Nur77 translocation pertaining to neuroprotection has not been investigated yet. The aim of our study was to perform a kinetic study on the effect of neurotoxic 6-hydroxydopamine (6-OHDA) and 17β-estradiol (E2) on the subcellular localization of Nur77 with reference to the modulation of apoptosis in PC12 cells. Our results demonstrate that E2 administration alone does not affect Nur77 cytoplasmic/nuclear ratio, mRNA levels, or apoptosis in PC12 cells. The neurotoxin 6-OHDA significantly enhances cytoplasmic localization of Nur77 after merely 3 h, while precipitating apoptosis. 6-OHDA also increases Nur77 transcription, which could partly explain the rise in cytoplasmic localization of the protein. Finally, treatment with both E2 and 6-OHDA delays Nur77 accumulation in the cytoplasm and delays cell death for a few hours in our cellular paradigm. Pre-treatment with E2 does not alter the increase in levels of Nur77 mRNA produced by 6-OHDA, suggesting that a raise in nuclear translocation is likely responsible for the stabilization of the cytoplasmic/nuclear ratio until 6 h. These results suggest an intriguing cooperation between E2 and Nur77 toward cellular fate guidance.fr
dc.languageengfr
dc.publisherSpringer New York LLCfr
dc.subjectNur77fr
dc.subject17b-Estradiolfr
dc.subject6-OHDA Afr
dc.subjectApoptosisfr
dc.subjectPC12fr
dc.subjectCellular translocationfr
dc.title17β-estradiol delays 6-OHDA-induced apoptosis by acting on Nur77 translocation from the nucleus to the cytoplasmfr
dc.typeCOAR1_1::Texte::Périodique::Revue::Contribution à un journal::Article::Article de recherchefr
dcterms.bibliographicCitationNeurotoxicity Research, Vol. 25 (1), 124–134 (2014)fr
dc.identifier.doi10.1007/s12640-013-9442-zfr
dc.identifier.pubmed24277157fr
dc.subject.rvmŒstradiolfr
dc.subject.rvmApoptosefr
dc.subject.rvmRécepteurs nucléaires (Biochimie)fr
dc.subject.rvmFacteurs de transcriptionfr
dc.subject.rvm6-hydroxydopaminefr
rioxxterms.versionVersion of Recordfr
rioxxterms.version_of_recordhttps://doi.org/10.1007/s12640-013-9442-zfr
rioxxterms.project.funder_nameNatural Sciences and Engineering Research Council of Canadafr
rioxxterms.project.funder_nameCanadian Institutes of Health Researchfr
rioxxterms.project.funder_nameFonds de Recherche du Québec - Santéfr
bul.rights.periodeEmbargoInfinifr
dc.audience.peerreviewOuifr
Collection:Articles publiés dans des revues avec comité de lecture

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