Expression of the alpha 5 integrin gene in corneal epithelial cells cultured on tissue-engineered human extracellular matrices

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dc.contributor.authorLake, Jennifer-
dc.contributor.authorZaniolo, Karine-
dc.contributor.authorGaudreault, Manon-
dc.contributor.authorCarrier, Patrick-
dc.contributor.authorDeschambault, Alexandre-
dc.contributor.authorBazin, Richard-
dc.contributor.authorGermain, Lucie-
dc.contributor.authorSalesse, Christian-
dc.contributor.authorGuérin, Sylvain-
dc.description.abstractThe integrin α5β1 plays a major role in corneal wound healing by promoting epithelial cell adhesion and migration over the fibronectin matrix secreted as a cellular response to corneal damage. Expression of α5 is induced when rabbit corneal epithelial cells (RCECs) are grown in the presence of fibronectin. Here, we examined whether α5 expression is similarly altered when RCECs or human corneal epithelial cells (HCECs) are grown on a reconstructed stromal matrix used as an underlying biomaterial. Mass spectrometry and immunofluorescence analyses revealed that the biomaterial matrix produced by culturing human corneal fibroblasts with ascorbic acid (ECM/35d) contains several types of collagens, fibronectin, tenascin and proteoglycans. Results from transfection of CAT/α5-promoter plasmids, Western blot and EMSA analyses indicated that ECM/35d significantly increase expression of α5 in HCECs as a result of alteration in the expression and DNA binding of the transcription factors NFI, Sp1, AP-1 and PAX6. The biological significance of this biomaterial substitute on the expression of the α5 gene may therefore contribute to better understand the function played by the α5β1 integrin during corneal wound
dc.publisherIPC Science and Technology Pressfr
dc.subjectTranscription factorsfr
dc.subjectExtracellular matrixfr
dc.subjectCorneal wound healingfr
dc.subjectTissue engineeringfr
dc.subjectBiomaterial substitutefr
dc.titleExpression of the alpha 5 integrin gene in corneal epithelial cells cultured on tissue-engineered human extracellular matricesfr
dc.title.alternativeExpression of the α5 integrin gene in corneal epithelial cells cultured on tissue-engineered human extracellular matricesfr
dc.typeCOAR1_1::Texte::Périodique::Revue::Contribution à un journal::Article::Article de recherchefr
dcterms.bibliographicCitationBiomaterials, Vol. 34 (27), 6367–6376 (2013)fr
dc.subject.rvmIntégrine alpha5fr
dc.subject.rvmSubstance fondamentale (Histologie)fr
dc.subject.rvmÉpithélium antérieur de la cornéefr
dc.subject.rvmExpression géniquefr
dc.subject.rvmCellules épithélialesfr
dc.subject.rvmCellules -- Culturefr
rioxxterms.versionVersion of Recordfr
rioxxterms.project.funder_nameCanadian Institutes of Health Researchfr
Collection:Articles publiés dans des revues avec comité de lecture

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